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主营:神经科学领域的蛋白及抗体
℡ 4000-520-616
℡ 4000-520-616
PhosphoSolutions/Raf-1 (Ser301) Antibody/p1555-301/100 µl
产品编号:p1555-301
市  场 价:¥4574.70
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$345.00
品      牌: PhosphoSolutions
公      司:PhosphoSolutions
公司分类:
PhosphoSolutions/Raf-1 (Ser301) Antibody/p1555-301/100 µl
商品介绍

Rabbitpolyclonalantibody

PooledSerum
Size:
100µl
Formulation:
AffinityPurifiedfromPooledSerum
Specificity:
Rat,Mouse,Human,Xenopus,Bovine,Chicken,Non-humanprimate,Canine
Applications:
WB1:1000
Species:
Rabbit
MolecularReference:
~74kDa
CiteThisAntibody:
PhosphoSolutionsCat#p1555-301,RRID:AB_2492223
Antigen/Purification:ExpandCollapse

Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser301ofratRaf-1.

Preparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-andnon-phosphopeptideaffinitycolumns.

BIOLOGicalSignificance:ExpandCollapse

TheRaspathwayisacriticalsignaltransductioncascadeinvolvedinregulatingcellularproliferation,differentiation,survival,andoncogenictransformation.MembersoftheRafserine/threoninekinasefamilyarekeyintermediatesinthiscascade,functioningtorelaysignalsfromactivatedRastothedownstreamproteinkinasesMEKandERK(Marshall,1996).PreviousstudieshaveshownthatphosphorylationisrequiredforRaf-1activation(DhillonandKolch,2002;Chongetal.,2003).RecentworkhasdemonstratedthatphosphorylationalsoregulatesthedownregulationofRaf(Doughertyetal.,2005)withtwositesparticipating:Ser301andSer642.

Storage

100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.

Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.

GeneralReferences

ChongH,VikisHG,GuanKL(2003)MechanismsofregulatingtheRafkinasefamily.CellularSignalling15:463-469.

DhillonAS,KolchW(2002)UntyingtheregulationoftheRaf-1kinase.ArchBiochemBiophys404:3-9.

DoughertyMK,MullerJ,RittDA,ZhouM,ZhouXZ,CopelandTD,ConradsTP,VeenstraTD,LuKP,MorrisonDK(2005)RegulationofRaf-1byDirectFeedbackPhosphorylation.MolCell17:215-224.

MarshallCJ(1996)Raseffectors.currentopinionincellbiology8:197-204.

品牌介绍

PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。

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