Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser642ofratRaf-1.
Theantibodyispreparedfromrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-andnon-phosphopeptideaffinitycolumns.
TheRaspathwayisacriticalsignaltransductioncascadeinvolvedinregulatingcellularproliferation,differentiation,survival,andoncogenictransformation.MembersoftheRafserine/threoninekinasefamilyarekeyintermediatesinthiscascade,functioningtorelaysignalsfromactivatedRastothedownstreamproteinkinasesMEKandERK(Marshall,1996).PreviousstudieshaveshownthatphosphorylationisrequiredforRaf-1activation(DhillonandKolch,2002;Chongetal.,2003).RecentworkhasdemonstratedthatphosphorylationalsoregulatesthedownregulationofRaf(Doughertyetal.,2005)withtwositesparticipating:Ser301andSer642.
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ChongH,VikisHG,GuanKL(2003)MechanismsofregulatingtheRafkinasefamily.CellularSignalling15:463-469.
DhillonAS,KolchW(2002)UntyingtheregulationoftheRaf-1kinase.ArchBiochemBiophys404:3-9.
DoughertyMK,MullerJ,RittDA,ZhouM,ZhouXZ,CopelandTD,ConradsTP,VeenstraTD,LuKP,MorrisonDK(2005)RegulationofRaf-1byDirectFeedbackPhosphorylation.MolCell17:215-224.
MarshallCJ(1996)Raseffectors.currentopinionincellbiology8:197-204.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。