Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser9ofGSK3β.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
Glycogensynthasekinase3(GSK3)isaserine/threoninekinasethatisinvolvedintheregulationofmanysignalingpathways.Todate,2isoformshavebeenidentified:GSK3αandGSK3β.Specifically,GSK3βhasbeenshowntoplayakeyinhibitoryroleinboththeinsulinandWntsignalingpathways(PapkoffandAikawa1998).IthasbeensuggestedthatSer9phosphorylationunderliestheinhibitionofGSK3βbyinsulin(Sutherlandetal.,1993).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
SutherlandC,LeightonIA,CohenP(1993)Inactivationofglycogensynthasekinase3betabyphosphorylation:newkinaseconnectionsininsulinandgrowthfactorsignaling.BiochemJ.Nov15;296(Pt.1):15-9.
PapkoffJ,AikawaM(1998)WNT-1andHGFregulateGSK3bactivityandbetacateninsignalinginmammaryepithelialcells.BiochemBiophysResCommun.Jun29;247(3):851-8.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。