Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Human,Bovine,Non-humanprimate,Canine
- Applications:
- WB1:1,000
- Species:
- Rabbit
- MolecularReference:
- ~45kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p180-292,RRID:AB_2492147
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr292ofhumanMEK1.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
MEK1(MAPkinasekinase,alsoknownasMKK)isanintegralcomponentoftheMAPkinasecascadethatregulatescellgrowthanddifferentiation(Ahn,1993;Chongetal.,2003).Thispathwayalsoplaysakeyroleinsynapticplasticityinthebrain(AdamsandSweatt,2002).ActivatedMEK1actsasadualspecificitykinasephosphorylatingbothathreonineandatyrosineresidueonMAPkinase(Kyriakisetal.,1991;Segeretal.,1991;Crewsetal.,1992).Conversely,therealsoappearstobeafeedbackphosphorylationofMEK1byMAPkinase.ThesitesonMEK1thatarephosphorylatedbyMAPkinaseareThr292andThr386(Mansouretal.,1994).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Tassin,T.C.,Benavides,D.R.,Plattner,F.,Nishi,A.,&Bibb,J.A.(2015).RegulationofERKKinasebyMEK1KinaseInhibitionintheBrain.JournalofBiologicalChemistry,Jun26;290(26):16319-29.PMID:25971971
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik，他们在 Paul Greengard 博士的实验室共同工作，Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区，是一支敬业、友好、勤奋的科学家团队，致力于为您提供最优质的抗体和业内客户支持。
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