Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser311,Thr315ofMEK5.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
MEK5(alsoknownasMKK5)isadualspecificityserine/threonineproteinkinasebelongingtotheMAPkinasekinasefamily.MEK5hasbeenshowntospecificallyactivateERK5(Zhouetal.,1995)whereasMEK5itselfisregulatedbyMEKK3(Chaoetal.,1999).AnimportantlinkbetweenMEK5andmetastaticprostratecancerhasbeendemonstrated(Mehtaet.al.,2003).DualphosphorylationofSer311andThr315havebeenimplicatedincellproliferation(Cameronetal.,2004).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ZhouG,BaoZQ,DixonJE,(1995)Componentsofanewhumanproteinkinasesignaltransductionpathway. J.Biol.Chem.270(21):12665-9.
ChaoTH,HayashiM,TappingRI,KatoY, LeeJD(1999)MEKK3directlyregulatesMEK5activityaspartofthebigmitogen-activatedproteinkinase1(BMK1)signalingpathway. J.Biol.Chem.274(51):36035-8.
MehtaPB,JenkinsBL,McCarthyL,ThilakL,RobsonCN,NealDE,LeungHY(2003)MEK5overexpressionisassociatedwithmetastaticprostratecancer,andstimulatesproliferation,MMP-9expressionandinvasion.Oncogene22(9):1381-9.
CameronSJ,AbeJ,MalikS,CheW,YangJ(2004)DifferentialroleofMEK5alphaandMEK5betainBMK1/ERK5activation.J.Biol.Chem.279(2):1506-12.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。