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主营:神经科学领域的蛋白及抗体
℡ 4000-520-616
℡ 4000-520-616
PhosphoSolutions/MEK5 (Ser311 Thr315) Antibody/p183-3115/100 µl
产品编号:p183-3115
市  场 价:¥4574.70
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$345.00
品      牌: PhosphoSolutions
公      司:PhosphoSolutions
公司分类:
PhosphoSolutions/MEK5 (Ser311 Thr315) Antibody/p183-3115/100 µl
商品介绍

Rabbitpolyclonalantibody

PooledSerum
Size:
100µl
Formulation:
AffinityPurifiedfromPooledSerum
Specificity:
Rat,Mouse,Human,Xenopus,Bovine,Chicken,Non-humanprimate,Zebrafish,Canine
Applications:
WB1:1,000
Species:
Rabbit
MolecularReference:
~49kDa
CiteThisAntibody:
PhosphoSolutionsCat#p183-3115,RRID:AB_2492149
Antigen/Purification:ExpandCollapse

Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser311,Thr315ofMEK5.

Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.

BIOLOGicalSignificance:ExpandCollapse

MEK5(alsoknownasMKK5)isadualspecificityserine/threonineproteinkinasebelongingtotheMAPkinasekinasefamily.MEK5hasbeenshowntospecificallyactivateERK5(Zhouetal.,1995)whereasMEK5itselfisregulatedbyMEKK3(Chaoetal.,1999).AnimportantlinkbetweenMEK5andmetastaticprostratecancerhasbeendemonstrated(Mehtaet.al.,2003).DualphosphorylationofSer311andThr315havebeenimplicatedincellproliferation(Cameronetal.,2004).

Storage

100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.

Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.

GeneralReferences

ZhouG,BaoZQ,DixonJE,(1995)Componentsofanewhumanproteinkinasesignaltransductionpathway. J.Biol.Chem.270(21):12665-9.

ChaoTH,HayashiM,TappingRI,KatoY, LeeJD(1999)MEKK3directlyregulatesMEK5activityaspartofthebigmitogen-activatedproteinkinase1(BMK1)signalingpathway. J.Biol.Chem.274(51):36035-8.

MehtaPB,JenkinsBL,McCarthyL,ThilakL,RobsonCN,NealDE,LeungHY(2003)MEK5overexpressionisassociatedwithmetastaticprostratecancer,andstimulatesproliferation,MMP-9expressionandinvasion.Oncogene22(9):1381-9.

CameronSJ,AbeJ,MalikS,CheW,YangJ(2004)DifferentialroleofMEK5alphaandMEK5betainBMK1/ERK5activation.J.Biol.Chem.279(2):1506-12.

品牌介绍

PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。

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