Rabbitpolyclonalantibody
PooledSerumTheantigenisapeptidefromtheC-terminalregion,specifictothehumanMnk2aisoform. ThesequenceoftheantigenhasnohomologywithMnk1orMnk2b.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthepeptideimmunogenwascoupled.
MAPkinase-interactingkinasesMnk1andMnk2areactivatedbytheMAPkinases,ERKandp38(Waskiewiczetal.,1997). Additionally,Mnk1andMnk2havebeenshowntophosphorylatethetranslationinitiationfactoreIF4E(FukunagaandHunter1997;Waskiewiczetal.,1997). AlternativesplicingoftheMKNK2generesultsintwoisoformswithdifferentC-termini:onethathasaMAPK-bindingdomain,Mnk2a,andonethatdoesnot,Mnk2b(Parraetal.,2005). RecentstudieshaveindicatedthatMnk2ahastumorsuppressiveactivitywhileMnk2bispro-oncogenic(Maimonetal.,2014).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Fukunaga,R.,&Hunter,T.(1997)ANewMAPKinase-ActivatedProteinKinaseIsolatedbyaNovelExpressionScreeningMethodtoIdentifyProteinKinaseSubstrates.EMBOJ.Apr15;16(8):1921-33.
Maimon A,MogilevskyM,ShiloA,Golan-GerstlR,ObiedatA,Ben-HurV,Lebenthal-LoingerI,SteinI,ReichR,BeenstockJ,ZehoraiE,AndersenCL,ThorsenK,OrntoftTF,DavisRJ,DavidsonB,MuD,KarniR(2014)Mnk2AlternativeSplicingModulatesthep38-MAPKPathwayandImpactsRas-InducedTransformation.CellReports7,501-513.
ParraJL,BuxadéM,ProudCG(2005)FeaturesoftheCatalyticDomainsandCTerminioftheMAPKSignal-integratingKinasesMnk1andMnk2DetermineTheirDifferingActivitiesandRegulatoryProperties.J.Biol.Chem.280,37623-37633.
WaskiewiczAJ,FlynnA,ProudCG,CooperJA(1997)Mitogen-activatedProteinKinasesActivatetheSerine/ThreonineKinasesMnk1andMnk2.EMBOJ.16,1909-1920.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。