Mousemonoclonalantibody
PooledSerumTheantigenisapeptidecorrespondingtoaminoacidresiduesfromtheC-terminalregionofratNSF,conjugatedtokeyholelimpethemocyanin(KLH).
TheantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviachromatographyonanaffinitycolumnmadewiththeC-terminalpeptideusedasantigen.
NSF(N-ethylmaleimidesensitivefusionprotein)isacriticalcomponentoftheSNARE(solubleNSFattachmentproteinreceptors)proteincomplexthatisinvolvedinsynapticvesicletrafficking.Specifically,NSFhasbeenfoundtobeessentialinmembranefusion.FurThermore,NSFhasbeenrecentlydemonstratedtobindotherproteincomplexessuchasAMPAreceptorsubunits(GluR2),GATE-16,LMA-1andRabssuggestingamorediverseroleintheassemblyofvariousproteincomplexes(Whiteheartetal.,2004).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
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KimCH,HahnMK,JoungY,AndersonSL,SteeleAH,Mazei-RobinsonMS,GizerI,TeicherMH,CohenBM,RobertsonD,WaldmanID,BlakelyRD,KimKS(2006)APolymorphisminthenorepinephrinetransportergenealterspromoteractivityandisassociatedwithattention-deficithyperactivitydisorder.ProcNat’lAcadSciUSA103(50):19164-9
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。