Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr180andphospho-Tyr182ofratp38MAPK.
Theantibodyis preparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
ThethreeMitogen-ActivatedProteinKinases(MAPKs)areevolutionarilyconservedproteinkinasesthatcontrolavastarrayofcellularprocesses.p38MAPKisoneofthesekinasesanditisactivatedbybothinflammatorycytokinesandbystress(JohnsonandLapadat,2002;ShiandGaestel,2002).Thep38MAPKisthoughttobeparticularlyimportantindiseaseslikeasthmaandautoimmunitybutitalsoplaysimportantrolesinthestressresponseofthenervoussystem(PhilipandArmstead,2003;Yingetal.,2002).LiketheotherMAPKs,p38isactivatedbyadualspecificitykinasethatphosphorylatesThr180andTyr182(Linetal.,1995).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
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Xu,P.,Zhou,Z.,Xiong,M.,Zou,W.,Deng,X.,Ganaie,S.S.,Kleiboeker,S.,Peng,J.,Liu,K.,Wang,S.andYe,S.Q.,2017.ParvovirusB19NS1proteininducescellcyclearrestatG2-phasebyactivatingtheATR-CDC25C-CDK1pathway.PLoSpathogens,13(3),p.e1006266.PMID:28264028
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。