RabbitPolyclonalAntibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Drosophila
- Applications:
- WB1:1000
- Species:
- Rabbit
- MolecularReference:
- ~70kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1705-398,RRID:AB_2629470
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr398ofDrosophila p70S6Kprotein.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphosphoandnon-phosphopeptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
p70S6kinase(p70S6K)isactivatedinasignalingpathwaythatincludesmTORand isamitogen-activatedSer/ThrproteinkinasethatisrequiredforcellgrowthandG1cellcycleprogression(Xioetal.,2009).p70S6Kiscontrolledbymultiplephosphorylationeventslocatedwithinthecatalytic,linkerandpseudosubstratedomainsandsubsequentlyphosphorylatesspecificallyribosomalproteinS6(Saitohetal.,2002).PhosphorylationofThr229inthecatalyticdomainandThr389inthelinkerdomainaremostcriticalforkinasefunction.Inhibitionofp70activityinhibitstheentryintoSphaseofthecellcycleandexhibitscellcyclearrestatG0/G1phase,suggestingthattheactivationofp70S6kplaysanobligatoryroleinmediatingmitogenicsignalsduringcellactivation(Xioetal.,2009).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ProductSpecificReferences
Tsokanos,F.F.,Albert,M.A.,Demetriades,C.,Spirohn,K.,Boutros,M.andTeleman,A.A.,2016.eIF4AinactivatesTORC1inresponsetoaminoacidstarvation.TheEMBOjournal,p.e201593118.PMID:26988032
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik，他们在 Paul Greengard 博士的实验室共同工作，Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区，是一支敬业、友好、勤奋的科学家团队，致力于为您提供最优质的抗体和业内客户支持。
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