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主营:神经科学领域的蛋白及抗体
℡ 4000-520-616
℡ 4000-520-616
PhosphoSolutions/Phospho-Thr356 PRAS40 Antibody/p204-356/100 µl
产品编号:p204-356
市  场 价:¥4574.70
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$345.00
品      牌: PhosphoSolutions
公      司:PhosphoSolutions
公司分类:
PhosphoSolutions/Phospho-Thr356 PRAS40 Antibody/p204-356/100 µl
商品介绍

Rabbitpolyclonalantibody

PooledSerum
Size:
100µl
Formulation:
AffinityPurifiedfromPooledSerum
Specificity:
Drosophila
Applications:
WB1:500
Species:
Rabbit
MolecularReference:
~60kDa
CiteThisAntibody:
PhosphoSolutionsCat#p204-356,RRID:AB_2492214
Antigen/Purification:ExpandCollapse

Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr356ofPRAS40.

Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.

BIOLOGicalSignificance:ExpandCollapse

PRAS40(proline-richAkt/PKBsubstrateof40kDa)actsattheintersectionoftheAkt-andmTOR-mediatedsignalingpathwaysanditsphosphorylationbyAktandbymTORC1resultsindissociationofPRAS40frommTORC1whichmayrelieveaninhibitoryconstraintonmTORC1activity(Wizaetal.,2012).PhosphorylationofPRAS40byAktandassociationwith14-3-3hasalsobeenindicatedtobecrucialforinsulintostimulatemTOR.(VanderHaaretal.,2007).TheprimaryfunctionofPRAS40invivoinDrosophilahasbeenshowntoregulateTORC1activity,andnottoactasadownstreamtargetandeffectorofTORC1(Pallaresetal.,2012).

Storage

100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.

Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.

GeneralReferences

WizaC,NascimentoEBM,andOuwensDM(2012).RoleofPRAS40inAktandmTORsignalinginhealthanddisease.AJPENDO.302:E1453–E1460.

VanderHaarE,LeeSI,BandhakaviS,GriffinTJ,KimDH.(2007).InsulinsignalingtomTORmediatedbytheAkt/PKBsubstratePRAS40.NatCellBiol3:316-23

PallaresC,Cakan-AkdoganG,andTelemanAA(2012).Tissue–SpecificCouplingbetweenInsulin/IGFandTORC1SignalingviaPRAS40inDrosophila.DevelopmentalCell.22:172-8

品牌介绍

PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。

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