Rabbitpolyclonalantibody
TheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephosphoSer482,486,490ofXenopuspolo-likekinasekinase(PLKK).
Theantibodyispreparedfromrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephosphopeptideaffinitycolumns.
Considerableevidenceindicatesthatapolo-likekinase(PLK)playsanimportantroleincellcycleregulation.PLKisalsorequiredforbipolarspindleformation,activationoftheanaphase-promotingcomplex/cyclosome,andcytokinesis.RecentworkledtotheidentificationofaPLKKthatisthoughttoberesponsIBLeforactivationofPLK.Recentwork(Erikson,etal.,2004)hasshownthatPLKKisinturnactivatedbyphosphorylationatthreesites(Ser482,Ser486andSer490).ThusactivationofPLKisthoughttoinvolveakinasecascadeinvolvingthephosphorylationofSer482,486,490inPLKK
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
EriksonE,HaysteadTAJ,Qian,Y-W,MallerJL(2004)Afeedbackloopinthepolo-likekinaseactivationpathway.JBiolChem279:32219-32224.
KumagaiA,DunphyWG(1996)PurificationandmolecularcloningofPlx1,acdc25-regulatorykinasefromXenopuseggextracts.Science273:1377-1380.LiuJ,LewellynAL,ChenLG,MallerJL(2004)ThepoloboxisrequiredformultiplefunctionsofPlx1inmitosis.JBiolChem279:21367-21373.
vanVugt,MATM,vandeWeerdt,BCM,VaderG,JanssenH,CalafatJ,KlompmakerR,WolthuisRMF,MedemaRH(2004)Polo-likekinase-1isrequiredforbipolarspindleformationbutisdispensableforanaphasepromotingcomplex/cdc20activationandinitiationofcytokinesis.JBiolChem279:36841-36854.
EleanorErikson,TimothyA.J.Haystead,Yue-WeiQian,andJamesL.Maller(2004)AFeedbackLoopinthePolo-likeKinaseActivationPathway.J.Biol.Chem.,Jul2004;279:32219–32224
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。