Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser459ofhumanPTPH1.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
Protein-tyrosinePhosphataseH1(PTPH1)hasrecentlybeenidentifiedasaspecificp38γMAPKphosphatasewhichismediatedthroughPDZinteraction(Houetal.,2010).Rashasbeendemonstratedtoincreasebothp38γandPTPH1proteinexpression,andthereisacouplingofincreasedp38γandPTPH1proteinexpressioninprimarycoloncancertissues(Houetal.,2010).PhosphorylationofPTPH1atSer459leadstoPTPH1stABIlization,whichplaysanimportantroleinRasoncogenesisandstressresponse(Houetal.,2012).Additionally,phosphorylationofPTPH1Ser459revealsanovelmechanismbywhichMAPKsignalsthroughPTPH1toregulatecellularresponse(Houetal.,2012).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
HouSW,SureshPS,QiX,LeppA,MirzaSP,ChenG(2012)p38γMitogen-activatedProteinKinaseSignalsthroughPhosphorylatingItsPhosphatasePTPH1inRegulatingRasProteinOncogenesisandStressResponse.JBiolChem287(33):27895-905.PMID:22730326
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。