Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser181ofTAO2.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
Invitro,TAO(thousandandoneaminoacid)proteinkinase2(TAO2)activatesMAP/ERKkinases(MEKs)3,4,and6towardtheirsubstratesp38MAPkinaseJNK/SAPK(Chenetal.,1999;ChenandCobb,2001).ThisandmorerecentworkhasledtotheproposalthattheTAOproteinkinasesplayanessentialroleinsignalingfromphysiologicalagoNISTstothestress-responsivep38MAPKs(Chenetal.,2003).AutophosphorylationofTAOmayplayaroleinthemechanismofTAOactivation.TheMEKbindingdomainofTAOisautophosphorylatedonbothserineandthreonineresiduesandSer181islocatedwithinthisdomain.
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
ChenZ,RamanM,ChenL,LeeSF,GilmanAG,CobbMH(2003)TAO(thousand-and-oneaminoacid)proteinkinasesmediatesignalingfromcarbacholtop38mitogen-activatedproteinkinaseandternarycomplexfactors.JBiolChem278:22278-22283.
ChenZ,Cobb,MH(2001)Regulationofstress-responsivemitogen-activatedprotein(MAP)kinasepathwaysbyTAO2.JBiolChem276:16070-16075.
ChenZ,HutchisonM,CobbMH(1999)IsolationoftheproteinkinaseTAO2andidentificationofitsmitogen-activatedproteinkinase/extracellularsignal-regulatedkinasekinasebindingdomain.JBiolChem274:28803-28807.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。