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主营:神经科学领域的蛋白及抗体
℡ 4000-520-616
℡ 4000-520-616
PhosphoSolutions/TAO2 (Ser181) Antibody/p275-181/100 µl
产品编号:p275-181
市  场 价:¥4574.70
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$345.00
品      牌: PhosphoSolutions
公      司:PhosphoSolutions
公司分类:
PhosphoSolutions/TAO2 (Ser181) Antibody/p275-181/100 µl
商品介绍

Rabbitpolyclonalantibody

PooledSerum
Size:
100µl
Formulation:
AffinityPurifiedfromPooledSerum
Specificity:
Rat,Mouse,Human,Xenopus,Zebrafish,Canine
Applications:
WB1:1,000
Species:
Rabbit
MolecularReference:
~120kDa
CiteThisAntibody:
PhosphoSolutionsCat#p275-181,RRID:AB_2492255
Antigen/Purification:ExpandCollapse

Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser181ofTAO2.

Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.

BIOLOGicalSignificance:ExpandCollapse

Invitro,TAO(thousandandoneaminoacid)proteinkinase2(TAO2)activatesMAP/ERKkinases(MEKs)3,4,and6towardtheirsubstratesp38MAPkinaseJNK/SAPK(Chenetal.,1999;ChenandCobb,2001).ThisandmorerecentworkhasledtotheproposalthattheTAOproteinkinasesplayanessentialroleinsignalingfromphysiologicalagoNISTstothestress-responsivep38MAPKs(Chenetal.,2003).AutophosphorylationofTAOmayplayaroleinthemechanismofTAOactivation.TheMEKbindingdomainofTAOisautophosphorylatedonbothserineandthreonineresiduesandSer181islocatedwithinthisdomain.

Storage

100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.

Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.

GeneralReferences

ChenZ,RamanM,ChenL,LeeSF,GilmanAG,CobbMH(2003)TAO(thousand-and-oneaminoacid)proteinkinasesmediatesignalingfromcarbacholtop38mitogen-activatedproteinkinaseandternarycomplexfactors.JBiolChem278:22278-22283.

ChenZ,Cobb,MH(2001)Regulationofstress-responsivemitogen-activatedprotein(MAP)kinasepathwaysbyTAO2.JBiolChem276:16070-16075.

ChenZ,HutchisonM,CobbMH(1999)IsolationoftheproteinkinaseTAO2andidentificationofitsmitogen-activatedproteinkinase/extracellularsignal-regulatedkinasekinasebindingdomain.JBiolChem274:28803-28807.

品牌介绍

PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。

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