Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser416ofrattau.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
Tauisakeymicrotubule-associatedproteinthatplaysanimportantroleintheformationofmicrotubulesinaxons(Binderetal.1985).SixtauisoformshavebeenidentifiedasproductsofasinglegeneproducedbyalternativemRNAsplicing(Goedert1990).TaumutationshavebeenimplicatedinmanyneurodegenerativedisorderssuchasAlzheimer’sdisease(AD),Pick’sdiseaseandprogressivesupranuclearpalsy.IthasbeenwelldocumentedthathyperphosphorylatedtauisamajorcomponentofpairedhelicalfilamentsinADbrain(Lee1995).Serine416hasbeendemonstratedtobeamajorphosphorylationsiteinvitrobyCaMkinaseII(Steineratal.1990).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
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GoedertM.andJakesR.(1990)Expressionofseparateisoformsofhumantauprotein:correlationwiththetaupatterninbrainandeffectsontubulinpolymerization.EMBOJ9,4225-4230.
SteinerB.,MandelkowE.M.,BiernatJ.etal.(1990)Phosphorylationofmicrotubuleassociatedproteintau:identificationofthesiteforCa2+-calmodulindependentkinaseandrelationshipwithtauphosphorylationinAlzheimertangles.EMBOJ.9,3539-3544.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。