Mousemonoclonalantibody
Theantigenisa PeptidecorrespondingtoaminoacidresiduesfromtheN-terminalregionofhumanthyroidhormonereceptor,α2-isotype.
TheantibodyispreparedfrommouseascitesbyammoniumsulfateprecipitationfollowedbyaffinitypurificationonaProteinGcolumn.
Thyroidhormonesareessentialfordevelopmentofthecentralnervoussystemanddeficitsinthesehormonesduringdevelopmentaffectssuchcognitivefunctionsaslearningandmemory(Ambroginietal.,2005;ChanandKilby,2000).Thyroidhormonesexerttheirphysiologicalrolemainlythroughbindingtospecificnuclearreceptorsincludingthepredominantisoformsofthyroidhormonereceptors,TRα1,TRα2,TRβ1andTRβ2.TRα1,TRβ1andTRβ2bindT3withhighaffinityandalsobindtothyroidhormoneresponseelements(TREs)onchromatintoregulatethetranscriptionalprocessesinseveraltargettissues,includingadultratbrain(Constantinouetal.,2005).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
AmbroginiP,CuppiniR,FerriP,ManciniC,CiaroniS,VociA,GerdoniE,GalloG(2005)Thyroidhormonesaffectneurogenesisinthedentategyrusofadultrat.Neuroendocrinology81:244-253.
ChanS,KilbyMD(2000)Thyroidhormoneandcentralnervoussystemdevelopment.JEndocrinol165:1-8.
ConstantinouC,MargarityM,ValcanaT(2005)Region-specificeffectsofhypothyroidismontherelativeexpressionofthyroidhormonereceptorsinadultratbrain.MolCellBiochem278:93-100.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。