Rabbitpolyclonalantibody
PooledSerumTheantigenisanativeproteinpurifiedfrombovinebrain.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthenativeproteinwascoupled.
SynapsinIplaysakeyroleinsynapticplasticityinbrain(Fengetal.,2002;Nayaketal.,1996).ThiseffectisdueinlargeparttotheABIlityofthesynapsinstoregulatetheavailabilityofsynapticvesiclesforrelease.Inadditiontoitsroleinplasticity,theexpressionofsynapsinIisapreciseindicatorofsynapseformation(MooreandBernstein,1989;Stoneetal.,1994).Thus,synapsinIimmunocytochemistryprovidesavaluabletoolforthestudyofsynaptogenesis.Theroleofsynapsininsynapticplasticityandinsynaptogenesisisregulatedbyphosphorylation(Jovanovicetal.,2001;Kaoetal.,2002).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Song,S.H.,&Augustine,G.J.(2016).SynapsinisoformsregulatingGABAreleasefromhippocampalinterneurons.TheJournalofNeuroscience,36(25),6742-6757.PMID:27335405
Wahba,G.,Hebert,A.E.,Grynspan,D.,Staines,W.,&Schock,S.(2016).Arapidandefficientmethodfordissociatedculturesofmousemyentericneurons.Journalofneurosciencemethods,261,110-116.PMID:26706461
Magupalli,V.G.,Mochida,S.,Yan,J.,Jiang,X.,Westenbroek,R.E.,Nairn,A.C.,Scheuer,T.&Catterall,W.A.(2013).Ca2+-independentactivationofCa2+/calmodulin-dependentproteinkinaseIIboundtotheC-terminaldomainofCaV2.1calciumchannels.JournalofBiologicalChemistry,288(7),4637-4648. PMID:23255606
KurtisD.Davies,SusanM.Goebel-Goody,StevenJ.Coultrap,andMichaelD.Browning(2008)LongTermSynapticDepressionThatIsAssociatedwithGluR1DephosphorylationbutNot-Amino-3-hydroxy-5-methyl-4-isoxazolepropionicAcid(AMPA)ReceptorInternalizationJ.Biol.Chem.,283:33138–33146.PMID:18819923
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。