Mousemonoclonalantibody
TheantigenisarecombinantproteincorrespondingtoaminoacidresiduesfromtheC-terminalregionofratsynaptojanin1.
TheantibodyisProteinGpurifiedculturesupernatant.
Synaptojaninisaphosphatidylinositolphosphataseinvolvedinclathrin-mediatedendocytosisofsynapticvesicles.Synaptojanin1hastwoalternativelysplicedisoforms;onethatis~145kDAandisexclusivelyexpressedinneurons,andtheother~170kDawhichisexpressedinnon-neuronal,peripheraltissues(RamjaunAR&McPhersonPS,1996).ThegenewhichencodesSynaptojanin1,SYNJ1,hasbeenmappedtochromosome21thusmakingitacandidateforinvolvementinDown’ssyndrome(DS).IthasrecentlybeendemonstratedthatTs65Dnmice(themostcommonlyusedmodelofDS)havealteredphosphatidylinositol-4,5-bisphosphatemetabolism.ThisdefectisrescuedbyrestoringSYNJ1todisomyintheTs65Dnmice(VoronovSVetal.,2008).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Tissue-specificalternativesplicinggeneratestwosynaptojaninisoformswithdifferentialmembranebindingproperties(1996).RamjaunAR,McPhersonPS.JBiolChem.Oct4;271(40):24856-61.
Synaptojanin1-linkedphosphoinositidedyshomeostasisandcognitivedeficitsinmousemodelsofDown’ssyndrome(2008).VoronovSV,FrereSG,GiovediS,PollinaEA,BorelC,ZhangH,SchmidtC,AkesonEC,WenkMR,CimasoniL,ArancioO,DavissonMT,AntonarakisSE,GardinerK,DeCamilliP,DiPaoloG.ProcNatlAcadSciUSA.Jul8;105(27):9415-20.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。