Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser58oftryptophanHydroxylase1(TPH1).
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
Tryptophanhydroxylase(TPH)catalyzesthe5-hydroxylationoftryptophan,whichisthefirststepinthebiosynthesisofindoleamines(SEROtoninandmelatonin)(Martinezetal.,2001).Inmammals,serotoninbiosynthesisoccurspredominantlyinneuronswhichoriginateintheRaphenucleiofthebrain,andmelatoninsynthesistakesplacewithinthepinealgland.AlthoughTPHcatalyzesthesamereactionwithintheRaphenucleiandthepinealgland,TPHactivityisrate-limitingforserotoninbutnotmelatoninbiosynthesis.Serotoninfunctionsmainlyasaneurotransmitter,whereasmelatoninistheprincipalhormonesecretedbythepinealgland.TheactivityofTPHisenhancedbyphosphorylationbycAMP-dependentproteinkinase(PKA)andCa2+/calmodulinkinaseII(CaMKII)(Jiangetal.,2000;Johansenetal.,1996)BothPKAandCaMKIIphosphorylateSer58whichlieswithintheregulatorydomainofTPH(Kuhnetal.,1997).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
JiangGC,YohrlingGJ,SchmittJD,VranaKE(2000)Identificationofsubstrateorientingandphosphorylationsiteswithintryptophanhydroxylaseusinghomology-basedmolecularmodeling.JMolBiol302:1005-1017.
JohansenPA,JenningsI,CottonRG,KuhnDM(1996)PhosphorylationandactivationoftryptophanhydroxylasebyexogenousproteinkinaseA.JNeuRochem66:817-823.
Kuhn,DM,Arthur,Jr,R,States,JC(1997)Phosphorylationandactivationofbraintryptophanhydroxylase:identificationofserine-58asasubstratesiteforproteinkinaseA.JNeurochem68:2220-2223.
MartinezA,KnappskogPM,HaavikJ(2001)Structuralapproachintohumantryptophanhydroxylaseanditsimplicationsfortheregulationofserotoninbiosynthesis.CurrMedChem8:1077-1091.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。