Rabbitpolyclonalantibody
PooledSerum- Size:
- 100µl
- Formulation:
- AffinityPurifiedfromPooledSerum
- Specificity:
- Rat,Mouse,Bovine,Zebrafish
- Applications:
- WB1:1,000
- Species:
- Rabbit
- GeneName:
- TPH2
- MolecularReference:
- ~55kDa
- CiteThisAntibody:
- PhosphoSolutionsCat#p1575-19,RRID:AB_2492272
Antigen/Purification:Collapse
Theantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser19ofrattryptophanhydroxylase2(TPH2).
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
BIOLOGicalSignificance:Collapse
Tryptophanhydroxylase(TPH)catalyzesthe5-hydroxylationoftryptophan,whichisthefirststepinthebiosynthesisofindoleamines(SEROtoninandmelatonin)(Martinezetal.,2001).Inmammals,serotoninbiosynthesisoccurspredominantlyinneuronswhichoriginateintheRaphenucleiofthebrain,andmelatoninsynthesistakesplacewithinthepinealgland.AlthoughTPHcatalyzesthesamereactionwithintheRaphenucleiandthepinealgland,TPHactivityisrate-limitingforserotoninbutnotmelatoninbiosynthesis.Serotoninfunctionsmainlyasaneurotransmitter,whereasmelatoninistheprincipalhormonesecretedbythepinealgland.TheactivityofTPHisenhancedbyphosphorylationbycAMP-dependentproteinkinase(PKA)andCa2+/calmodulinkinaseII(CaMKII)(Jiangetal.,2000;Johansenetal.,1996).CaMKIIphosphorylatesSer19whichlieswithintheregulatorydomainofTPH2(McKinneyetal.,2005).
Storage
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
GeneralReferences
JiangGC,YohrlingGJ,SchmittJD,VranaKE(2000)Identificationofsubstrateorientingandphosphorylationsiteswithintryptophanhydroxylaseusinghomology-basedmolecularmodeling.JMolBiol302:1005-1017.
JohansenPA,JenningsI,CottonRG,KuhnDM(1996)PhosphorylationandactivationoftryptophanhydroxylasebyexogenousproteinkinaseA.JNeuRochem66:817-823.
MartinezA,KnappskogPM,HaavikJ(2001)Structuralapproachintohumantryptophanhydroxylaseanditsimplicationsfortheregulationofserotoninbiosynthesis.CurrMedChem8:1077-1091.
McKinneyJ,KnappskogPM,HaavikJ(2005)Differentpropertiesofthecentralandperipheralformsofhumantryptophanhydrpxylase.JNeurochem92(2):311-20.
KuhnDM,SakowskiSA,GeddesTJ,WilkersonC,HaycockJW(2007)Phosphorylationandactivationoftryptophanhydroxylase2:identificationofserine-19asthesubstratesiteforcalcium,calmodulin-dependentproteinkinaseII.JNeurochem103(4):1567-73.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik，他们在 Paul Greengard 博士的实验室共同工作，Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区，是一支敬业、友好、勤奋的科学家团队，致力于为您提供最优质的抗体和业内客户支持。
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