Mousemonoclonalantibody
TheantigenisasyntheticpeptidecorrespondingtobetaIIItubulin.
TheantibodyisProteinGpurifiedculturesupernatant.
Tubulinisthemajorconstituentofmicrotubules,existingasaheterodimeroftheαandβsubunits.ThebetaIIIisoformoftubulinisfoundalmostexclusivelyinneuronalprocessesofadulttissuesandisthereforeanexcellentMarkerforneurons.Neuronspecific,posttranslationalmodificationswithintheC-terminaldomainofbetaIIItubulinhavebeenshowntobedevelopmentallyregulatedsuggestingthattheymayservetomodulatetheinteractionoftubulinwithmicrotubuleassociatedproteins(Leeetal.,1990).Additionally,betaIIItubulinhasbeenfoundtobehighlyexpressedincancercellssuchassmallcelllungcancer,largecellneuroendocrinecarcinomaandadenocarcinomasandiscorrelatedwithanincreasinghistologicaldegreeofmalignancy(Katsetosetal.,2003)
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommendedinundilutedaliquots.Stableat–20°Cforatleast1year.Avoidfreeze/thawcycles.
Ka,M.,&Kim,W.Y.(2015).Microtubule-ActinCrosslinkingFactor1IsRequiredforDendriticArborizationandAxonOutgrowthintheDevelopingBrain.Molecularneurobiology,November,1-15.PMID:26526844.
Cooper,R.J.,&Spitzer,N.(2015).SilvernanoparticlesatsublethalconcentrationsdisruptCytoskeletonandneuritedynamicsinculturedadultneuralstemcells.Neurotoxicology,48,231-238.PMID:25952507.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。