Rabbit polyclonal antibody
Pooled Serum- Size:
- 100 µl
- Formulation:
- Affinity Purified from Pooled Serum
- Specificity:
- Bovine, Chicken, Guinea Pig, Human, Mouse, Non-human primate, Rat, Sheep
- Applications:
- WB 1:1000
- Species:
- Rabbit
- Molecular Reference:
- ~ 51 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# p1208-222, RRID:AB_2572427
Antigen/Purification: Collapse
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Ser222 of the human MEF2C protein.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Biological Significance: Collapse
MEF2C, also known as MADS box transcription enhancer factor 2, polypeptide C, is one of 4 MEF2 (myocyte enhancer factor 2) transcription factors that encode proteins for development of skeletal muscle and brain proliferation and differentiation (McDermott et al, 1993), along with regulating stress-response during cardiac hypertrophy in mammals (Wu et al, 2015). Phosphorylation of MEF2C at serine 59 has been shown to be negatively regulated by integrin-linked kinase (ILK) (Dong et al, 2015). Recently, high MEF2C expression has been associated with a subset of acute myeloid leukemia (AML) patients with adverse-risk disease features and poor outcomes (Laszlo et al, 2015). Phosphorylation of MEF2C at serine 222 may play a key role in MEF2C signaling.
Storage
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.
Product Specific References
Brown, F., Still, E., Cifani, P., Takao, S., Reed, C., Ficarro, S., Koche, R., Romanienko, P., Mark, W., O’Donnell, C., Spitzer, B., Stutzke C., Krivtsov, A., Pouliot, G., Gray, N., Marto, J., Armstrong, S., and Kentsis, A. 2017. MEF2C phosphorylation is required for chemotherapy resistance in acute myeloid leukemia. bioRxiv, p.107201.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik，他们在 Paul Greengard 博士的实验室共同工作，Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区，是一支敬业、友好、勤奋的科学家团队，致力于为您提供最优质的抗体和业内客户支持。
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