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主营:神经科学领域的蛋白及抗体
℡ 4000-520-616
℡ 4000-520-616
PhosphoSolutions/Nicotinic Acetylcholine Receptor (nAChR) β4 Antibody/1471-nACB4/100 µl
产品编号:1471-nACB4
市  场 价:¥4574.70
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$345.00
品      牌: PhosphoSolutions
公      司:PhosphoSolutions
公司分类:
PhosphoSolutions/Nicotinic Acetylcholine Receptor (nAChR) β4 Antibody/1471-nACB4/100 µl
商品介绍

RabbitPolyclonalAntibody

PooledSerum
Size:
100µl
Formulation:
AffinityPurifiedfromPooledSerum
Specificity:
Mouse,Rat
Applications:
WB1:1000
Species:
Rabbit
MolecularReference:
~52kDa
CiteThisAntibody:
PhosphoSolutionsCat#1471-nACB4,RRID:AB_2619714
Antigen/Purification:ExpandCollapse

Theantigenisafusionproteinfromthecytoplasmicloopoftheβ4-subunitofratnAChR.

Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthefusionproteinimmunogenwascoupled.

BIOLOGicalSignificance:ExpandCollapse

Nicotinicacetylcholinereceptors(nAChRs)areionotropic,cholinergicreceptorsthataredividedinto2types;muscletypeandneuronaltype.NeuronalnAChRsarepentamericionchannelsconsistingof5identical(homopentamers)ordifferent(heteropentamers)subunits.HeteropentamericneuronalnAChRsmediatefastsynaptictransmissionintheautonomicnervoussystem.Thepredominanthetero-oligomericnAChRintheCNScontainthesubunitsα4β2,whereasα3β4prevailinthePNS.However,theexpressionofthesesubunitsvariesnotonlybyregionbutalsoduringdevelopment(Scholzeetal2011).Inthebrain,β2-containingreceptorsgreatlyoutnumberreceptorsthatcontainβ4(McGehee&Role,1995;Albuquerqueetal.,2009),andinmostbrainregions,targeteddeletionoftheβ2subunitvirtuallyabolishes[3H]-epibatidinebindingandreceptorautorADIography(Zolietal.,1998)duetotheabsenceofaβsubunitrequiredtoformfunctionalnAChRs(Champtiaux&Changeux,2004).

Storage

100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.

Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.

GeneralReferences

Scholze,P.,Ciuraszkiewicz,A.,Groessl,F.,Orr‐Urtreger,A.,McIntosh,J.M.,&Huck,S.(2011).α4β2nicotinicacetylcholinereceptorsintheearlypostnatalmousesuperiorcervicalganglion.Developmentalneurobiology,71(5),390-399.

McGehee,D.S.,&Role,L.W.(1995).Physiologicaldiversityofnicotinicacetylcholinereceptorsexpressedbyvertebrateneurons.Annualreviewofphysiology,57(1),521-546.

Albuquerque,E.X.,Pereira,E.F.,Alkondon,M.,&Rogers,S.W.(2009).Mammaliannicotinicacetylcholinereceptors:fromstructuretofunction.Physiologicalreviews,89(1),73-120.

Zoli,M.,Léna,C.,Picciotto,M.R.,&Changeux,J.P.(1998).Identificationoffourclassesofbrainnicotinicreceptorsusingβ2mutantmice.TheJournalofneuroscience,18(12),4461-4472.

Champtiaux,N.,&Changeux,J.P.(2004).Knockoutandknockinmicetoinvestigatetheroleofnicotinicreceptorsinthecentralnervoussystem.Progressinbrainresearch,145,233-251.

品牌介绍

PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。

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