Sheeppolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser774ofratdynamin.
Theantibodyispreparedfrompooledsheepserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
Dynaminisamemberofagroupofnerveterminalproteinscalleddephosphinsthatregulatesynapticvesicleendocytosis(Cousinetal.,2001;Grahametal.,2002;Tsuboietal.,2002).Cyclindependentproteinkinase5phosphorylatesdynaminatSer774andSer778 thatarethephosphorylationsitesondynaminphosphorylatedinvivo(Tanetal.,2003).PhosphorylationofSer774byGSK3hasrecentlybeenshowntocontrolactivity-dependentbulkendocytosisofsynapticvesicles(Claytonetal.,2010).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Vargas,K.J.,Schrod,N.,Davis,T.,Fernandez-BusnADIego,R.,Taguchi,Y.V.,Laugks,U.,Lucic,V.andChandra,S.S.,2017.SynucleinsHaveMultipleEffectsonPresynapticArchitecture.CellReports,18(1),pp.161-173. PMID:28052246
SergioLeal-Ortiz,ClarissaL.Waites,RyanTerry-Lorenzo,PedroZamorano,EckartD.Gundelfinger,andCraigC.Garner(2008)PiccolomodulationofSynapsin1adynamicsregulatessynapticvesicleexocytosis.J.CellBiol.,181:831–846.PMID:18519737
TanTC,ValovaVA,MalladiCS,GrahamME,BervenLA,JuppOJ,HansraG,McClureSJ,SarcevicB,BoadleRA,LarsenMR,CousinMA,RobinsonPJ(2003)cdk5isessentialforsynapticvesicleendocytosis.NatCellBiol5:701-710. PMID:12855954
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。