Rabbitpolyclonalantibody
PooledSerumTheantigenisapeptidecorrespondingtoaminoacidresiduesfromtheN-terminalregionofratRab3,conjugatedtokeyholelimpethemocyanin(KLH).
Theantibodyis preparedfrompooledrabbitserumbyaffinitypurificationviachromatographyonanaffinitycolumnmadewiththeN-terminalpeptideusedasantigen.
RabproteinsbelongtotheRassmallGTPasesuperfamily.TheGTP-bindingRab3subfamilyhas4knownisoforms:3A,3B,3C,and3D(Schluteretal.,2004).Theseproteinsareexpressedinhighestconcentrationsinbrainandendocrinetissuesandinvolvedinregulatedexocytosisofhormonesandneurotransmitters(Coppolaetal.,2002).Specifically,Rab3’sarethoughttoplayakeyroleinpresynapticvesicletraffickingandpriming(Schluteretal.,2006).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
SchluterOM,SchmitzF,JahnR,RosenmundC,SudhofTC(2004)ACompleteGeneticAnalysisofNeuronalRab3Function.JNeuroscience24(29):6629-6637
SchluterOM,BasuJ,SudhofTC,RosenmundC(2006)Rab3SuperprimesSynapticVesiclesforRelease:ImplicationsforShort-termSynapticPlasticity.JNeuroscience26(4):1239-1246.
CoppolaT,Perret-MenoudV,GattescoS,MagninS,PomboI,BlankU,RegazziR(2002)ThedeathdomainofRab3guaninenucleotideexchangeproteininGDP/GTPexchangeactivityinlivingcells.JBiochem362(Pt.2):273-9.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。