Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser234ofrabphilin3A.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
Rabphilin-3AisaperipheralmembraneproteinthatbindsCa2+andphospholipidsandisattachedtosynapticvesiclemembranes(Geppertetal.,1994;Lietal.,1994).Theexpressionofrabphilin3AisreducedinananimalmodelofHuntington’sdisease(Smithetal.,2005).RabphilincanbephosphorylatedatSer234bybothCAMkinaseIIandPKAandthisphosphorylationhasbeensuggestedtoregulateneuronalactivityduringdevelopmentinasynapse-specificmanner(Fykseetal.,1995;FolettiandScheller,2001).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Stanic,J.,Mellone,M.,Napolitano,F.,Racca,C.,Zianni,E.,Minocci,D.,Ghiglieri,V.,Thiolat,M.L.,Li,Q.,Longhi,A.andDeRosa,A.,2017.Rabphilin3A:Anoveltargetforthetreatmentoflevodopa-induceddyskinesias.NeurobiologyofDisease.PMID:28823933
Leal-Ortiz,S.,Waites,C.L.,Terry-Lorenzo,R.,Zamorano,P.,Gundelfinger,E.D.andGarner,C.C.,2008.PiccolomodulationofSynapsin1adynamicsregulatessynapticvesicleexocytosis.TheJournalofcellbiology,181(5),pp.831-846. PMID:18519737
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。