Rabbitpolyclonalantibody
PooledSerumTheantigenisasyntheticpeptidecorrespondingtoaminoacidresiduesfromtheC-terminalregionofrabphilin3A.
Theantibodyis preparedfrompooledrabbitserumbyaffinitypurificationonacolumntowhichtheC-terminalpeptideimmunogenwascoupled.
Rabphilin-3AisaperipheralmembraneproteinthatbindsCa2+andphospholipidsandisattachedtosynapticvesiclemembranes(Geppertetal.,1994;Lietal.,1994).Theexpressionofrabphilin3AisreducedinananimalmodelofHuntington’sdisease(Smithetal.,2005).RabphilincanbephosphorylatedatSer234bybothCAMkinaseIIandPKAandthisphosphorylationhasbeensuggestedtoregulateneuronalactivityduringdevelopmentinasynapse-specificmanner(Fykseetal.,1995;FolettiandScheller,2001).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
FolettiDL,SchellerRH(2001)Developmentalregulationandspecificbraindistributionofphosphorabphilin.JNeurosci21:5461-5472.
FykseEM,LiC,SudhofTC(1995)Phosphorylationofrabphilin-3AbyCa2+/calmodulin-andcAMPdependentproteinkinasesinvitro.JNeurosci15:2385-2395.
GeppertM,BolshakovVY,SiegelbaumSA,TakeiK,DeCamilliP,HammerRE,SüdhofTC(1994)TheroleofRab3Ainneurotransmitterrelease.Nature(Lond)369:493-497.
LiC,TakeiK,GeppertM,DaniellL,SteniusK,ChapmanER,JahnR,DeCamilliP,SüdhofTC(1994)Synaptictargetingofrabphilin-3A,asynapticvesicleCa2+/phospholipid-bindingprotein,dependsonrab3A/3C.Neuron13:885-898.
SmithR,PetersenA,BatesGP,BrundinP,LiJY(2005)Depletionofrabphilin3Ainatransgenicmousemodel(R6/1)ofHuntington’sdisease,apossIBLeculpritinsynapticdysfunction.NeurobiolDis20:673-684.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。