Chicken polyclonal antibody
- Size:
- 100 µl
- Formulation:
- Chicken, total IgY fraction
- Specificity:
- Rat, Mouse, Human
- Applications:
- WB 1:1,000 IF 1:500
- Species:
- Chicken
- Molecular Reference:
- ~50 kDa
- Cite This Antibody:
- PhosphoSolutions Cat# 2105-VIM, RRID:AB_2492283
Antigen/Purification: Collapse
The antigen is a recombinant human Vimentin purified from E. coli.
The antibody is Total IgY fraction.
Biological Significance: Collapse
Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006). Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008).
Storage
Liquid. Contains 10 mM sodium azide.
For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year. Avoid freeze/thaw cycles.
Product Specific References
Kirane, A., Ludwig, K. F., Sorrelle, N., Haaland, G., Sandal, T., Ranaweera, R., Toombs, J.E., Wang, M., Dineen, S.P., Micklem, D., Delliger, M.T., Lorens, J.B., & Brekken, R. A. (2015). Warfarin blocks Gas6-mediated Axl activation required for pancreatic cancer epithelial plasticity and metastasis. Cancer Research, canres-2887. PMID: 26206560
Ostapoff, K. T., Cenik, B. K., Wang, M., Ye, R., Xu, X., Nugent, D., Hagopian, M.M., Topalovski, M., Rivera, L.B., Carroll, K.D., & Brekken, R. A. (2014). Neutralizing murine TGFβR2 promotes a differentiated tumor cell phenotype and inhibits pancreatic cancer metastasis. Cancer research, 74(18), 4996-5007. PMID: 25060520
Kirane A, Toombs JE, Ostapoff K, Carbon JG, Zaknoen S, Braunfeld J, Schwarz RE, Burrows FJ, Brekken RA (2012) Apricoxib, a novel inhibitor of COX-2, markedly improves standard therapy response in molecularly defined models of pancreatic cancer. Clin Cancer Res. Sep 15;18(18):5031-42. PMID: 22829202
Kirane A, Toombs JE, Larsen JE, Ostapoff K, Meshaw KR, Zaknoen S, Brekken RA, Burrows FJ (2012) Epithelial-mesenchymal transition increases tumor sensitivity to COX-2 inhibition by apricoxib. Carcinogenesis 33(9):1639-46. PMID: 22678114
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik，他们在 Paul Greengard 博士的实验室共同工作，Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区，是一支敬业、友好、勤奋的科学家团队，致力于为您提供最优质的抗体和业内客户支持。
本网站将在规定时间内给予删除等相关处理。
