Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr1007ofmouseKCC2.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-andnon-phosphopeptideaffinitycolumns.
KCC2iswidelythoughttobeexpressedexclusivelyinneuronswhereitisresponsIBLeformaintaininglowintracellularchlorideconcentrationtodrivehyperpolarizingpost-synapticresponsestotheinhibitoryneurotransmittersGABAandglycine(Leeetal.,2007).KCC2isexpressedinmostadultneurons,andexpressionlevelscorrelatewellwiththematurationstateofneurons.N-ethylmaleimide(NEM)hasbeenshowtoincreasephosphorylationoftheSer940residue,whiledecreasingphosphorylationofT1007residue.DephosphorylationofresiduesT906andT1007correlateswithincreasedKCC2activity(Mossetal.,2017).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Conway,L.C.,Cardarelli,R.A.,Moore,Y.E.,Jones,K.,McWilliams,L.J.,Baker,D.J.,Burnham,M.P.,Bürli,R.W.,Wang,Q.,Brandon,N.J.andMoss,S.J.,2017.N-ethylmaleimideincreasesKCC2activitybymodulatingtransporterphosphorylation.JournalofBiologicalChemistry,pp.jbc-M117.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。