Rabbitpolyclonalantibody
PooledSerumTheantigenisapeptidecorrespondingtoaminoacidresiduesfromtheC-terminalregionofratSNAP25conjugatedtokeyholelimpethemocyanin(KLH).
TheantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviachromatographyonanaffinitycolumnmadewiththeC-terminalpeptideusedasantigen.
SNAP25(Synaptosomalassociatedproteinof25kDa)isapresynapticplasmamembraneproteinthatiswidelydistributedthroughoutthebrainandinvolvedintheregulationofneurotransmitterrelease.DecreasedlevelsofSNAP25havebeenfoundinthebrainsofpatientswithDownSyndromeandAlzheimer’sDisease(Greberetal.,1999).Inaddition,asignificantreductioninthehippocampalexpressionofSNAP25hasalsobeenfoundinpatientswithSchizophrenia(Fatemietal.,2001).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
FatemiSH,SidwellR,KistD,AkhterP,MeltzerHY,BaileyK,ThurasP,SedgwickJ(1998)Differentialexpressionofsynaptosome-associatedprotein25kDa(Snap25)inhippocampiofneonatalmicefollowingexposuretohumaninfluenzavirusinutero.BrainRes800(1):1-9.
GreberS,CairnsN,LubecG,FountoulakisM(1999)Decreasedlevelsofthesynaptosomalassociatedprotein25inthebrainsofpatientswithDownSyndromeandAlzheimer’sDisease.Electrophoresis20(4-5):928-34.
AndoK,KudoY,TakahashiM(2005)Negativeregulationofneurotransmitterreleasebycalpain:apossIBLespecificinvolvementofSnap25cleavage.JNeuRochem94(3):651-8.
FatemiSH,EarleJA,StaryJM,LeeS,SedgwickJ(2001)AlteredlevelsofthesynaptosomalassociatedproteinSnap25inhippocampusofpatientswithmooddisordersandSchizophrenia.Neuroreport.12(15):3257-62.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。