Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser549ofratsynapsinI.
Theantibodyis preparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns.
SynapsinIplaysakeyroleinsynapticplasticityinbrain(Fengetal.,2002;Nayaketal.,1996).ThiseffectisdueinlargeparttotheABIlityofthesynapsinstoregulatetheavailabilityofsynapticvesiclesforrelease.Theroleofsynapsininsynapticplasticityandinsynaptogenesisisregulatedbyphosphorylation(Jovanovicetal.,2001;Kaoetal.,2002).Ser549alongwithSer62andSer67arethesitesofsynapsinIthatarephosphorylatedbyMAPkinase(Jovanovicetal.,1996).Phosphorylationandsubsequentdephosphorylationofthissiteisthoughttoplayakeyroleinsynapticvesicletrafficking.
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
JovanovicJN,Benfenati,F,SiowYL,SihraTS,SangheraJS,PelechSL,GreengardP,CzernikAJ(1996)NeurotrophinsstimulatephosphorylationofSynapsinIbyMAPkinaseandregulateSynapsinI-actininteractions.Neurobiology93:3679-3683.
CzernikAJ,PangDT,GreengardP(1987)AminoacidsequencessurroundingthecAMP-dependentandcalcium/calmodulin-dependentphosphorylationsitesinratandbovinesynapsinI.ProcNatlAcadSci(USA)84:7518-7522.
FengJ,ChiP,BlanpiedTA,XuYM,MagarinosAM,FerreiraA,TakahashiRH,KaoHT,McEwenBS,RyanTA,AugustineGJ,GreengardP(2002)RegulationofneurotransmitterreleasebysynapsinIII.JNeurosci22:4372-4380.
JovanovicJN,SihraTS,NairnAC,HemmingsHC,Jr.,GreengardP,CzernikAJ(2001)OpposingchangesinphosphorylationofspecificsitesinsynapsinIduringCa2+-dependentglutamatereleaseinisolatednerveterminals.JNeurosci21:7944-7953.
KaoHT,SongHJ,PortonB,MingGL,HohJ,AbrahamM,CzernikAJ,PieriboneVA,PooMM,GreengardP(2002)AproteinkinaseA-dependentmolecularswitchinsynapsinsregulatesneuriteoutgrowth.NatureNeurosci5:431-437.
NayakAS,MooreCI,BrowningMD(1996)CAMkinaseIIphosphorylationofthepresynapticproteinsynapsinispersistentlyincreasedduringexpressionoflong-termpotentiation.ProcNatlAcadSci(USA)93:15451-15456.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。