Rabbitpolyclonalantibody,neatserum
Theantigenisanativeproteinpurifiedfrombovinebrain.
Theantibodyisunpurifiedneatserum.
SynapsinIplaysakeyroleinsynapticplasticityinthebrain(Fengetal.,2002;Nayaketal.,1996).ThiseffectisdueinlargeparttotheABIlityofthesynapsinstoregulatetheavailabilityofsynapticvesiclesforrelease.Inadditiontoitsroleinplasticity,theexpressionofsynapsinIisapreciseindicatorofsynapseformation(MooreandBernstein,1989;Stoneetal.,1994).Thus,SynapsinIimmunocytochemistryprovidesavaluabletoolforthestudyofsynaptogenesis.Theroleofsynapsininsynapticplasticityandinsynaptogenesisisregulatedbyphosphorylation(Jovanovicetal.,2001;Kaoetal.,2002).
50μlneatserum.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
FengJ,ChiP,BlanpiedTA,XuYM,MagarinosAM,FerreiraA,TakahashiRH,KaoHT,McEwenBS,RyanTA,AugustineGJ,GreengardP(2002)RegulationofneurotransmitterreleasebysynapsinIII.JNeurosci22:4372-4380.
JovanovicJN,SihraTS,NairnAC,HemmingsHC,Jr.,GreengardP,CzernikAJ(2001)OpposingchangesinphosphorylationofspecificsitesinsynapsinIduringCa2+-dependentglutamatereleaseinisolatednerveterminals.JNeurosci21:7944-7953.
KaoHT,SongHJ,PortonB,MingGL,HohJ,AbrahamM,CzernikAJ,PieriboneVA,PooMM,GreengardP(2002)AproteinkinaseA-dependentmolecularswitchinsynapsinsregulatesneuriteoutgrowth.NatureNeurosci5:431-437.
MooreRY,BernsteinM(1989)SynaptogenesisintheratsuprachiasmaticnucleusdemonstratedbyelectronmicroscopyandsynapsinIimmunoreactivity.JNeurosci9:2151-2162.
NayakAS,MooreCI,BrowningMD(1996)CaMkinaseIIphosphorylationofthepresynapticproteinsynapsinispersistentlyincreasedduringexpressionoflong-termpotentiation.ProcNatlAcadSci(USA)93:15451-15456.
StoneLM,BrowningMD,FingerTE(1994)Differentialdistributionofthesynapsinsintheratolfactorybulb.JNeurosci14:301-309.
Note:Dr.MichaelBrowningandDr.AndrewCzernik,co-authorsofthecitedpapersarethePresidentandChiefScientificOfficerofPhosphoSolutionsandtwoofitsfounders.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。