Mousemonoclonalantibody
Theantigenisasynapticimmunoprecipitate(crude)fromhumanbrain.
TheantibodyisProteinGpurifiedculturesupernatant.
Synaptobrevin(akaVAMP)isanintegralmembraneproteinofsynapticvesiclesthatplaysamajorroleintheformationoflargerSNAREcomplexes,alongwithSNAP-25andsyntaxin.Synaptobrevinhasbeenshowntobeessentialfortwofastsynapse-specificmembranetraffickingprocesses:fastexocytosisforneurotransmitterreleaseandfastendocytosisthatmediatesrapidrecyclingofsynapticvesicles(Deaketal.,2004).DecreasedlevelsofsynaptobrevininhumanhippocampusandcortexhavebeencorrelatedwithcognitivedeficitsinAlzheimer’sdisease(Szeetal.,2000).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
DeákF,SchochS,LiuX,SüdhofTC,KavalaliET(2004)Synaptobrevinisessentialforfastsynaptic-vesicleendocytosis.NatCellBiol.2004Nov;6(11):1102-8.
SzeCI,BiH,Kleinschmidt-DeMastersBK,FilleyCM,MartinLJ(2000)SelectiveregionallossofexocytoticpresynapticvesicleproteinsinAlzheimer’sdiseasebrains.JNeurolSci.175(2):81-90.
Honer,W.G.,Hu,L.&Davies,P.Humansynapticproteinswithaheterogeneousdistributionincerebellumandvisualcortex(1993).BrainResearch609,9-20.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。