Rabbitpolyclonalantibody
PooledSerumTheantigenisaphosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Thr202ofsynaptotagmin.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationviasequentialchromatographyonphospho-anddephospho-peptideaffinitycolumns
Synaptotagminiswidelyregardedastheprimarycalciumsensorforsynapticvesicleexocytosis(Fernandez-Chaconetal.,2001;Wangetal.,2003).Moreover,recentstudiesindicatethattheproteinalsoplaysakeyroleinendocytosis(Poskanzeretal.,2003).Synaptotagmincanbephosphorylatedbymultipleproteinkinasesandthismayplayakeyroleinmodulationofsynaptotagmin’sABIlitytoinfluenceboththeexocytoticandendocytoticcomponentsofsynaptictransmission(Hilfikeretal.,1999;Leeetal.,2004).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
Fernandez-ChaconR,KonigstorferA,GerberSH,GarciaJ,MatosMF,StevensCF,BroseN,RizoJ,RosenmundC,SudhofTC(2001)SynaptotagminIfunctionsasacalciumregulatorofreleaseprobability.Nature(London)410:41-49.
HilfikerS,PieriboneVA,NordstedtC,GreengardP,CzernikAJ(1999)RegulationofsynaptotagminIphosphorylationbymultipleproteinkinases.JNeuRochem73:921-932.
LeeBH,MinX,HeiseCJ,XuBE,ChenS,ShuH,Luby-PhelpsK,GoldsmithEJ,CobbMH(2004)WNK1phosphorylatessynaptotagmin2andmodulatesitsmembranebinding.MolCell15:741-751.
PoskanzerKE,MarekKW,SweeneyST,DavisGW(2003)SynaptotagminIisnecessaryforcompensatorysynapticvesicleendocytosisinvivo.Nature(London)426:559-563.
WangCT,LuJC,BaiJH,ChangPY,MartinTFJ,ChapmanER,JacksonMB(2003)Differentdomainsofsynaptotagmincontrolthechoicebetweenkiss-and-runandfullfusion.Nature(London)424:943-947.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。