Rabbitpolyclonalantibody
PooledSerumTheantigenisasyntheticpeptidecorrespondingtoaminoacidresidueswithinthesynaptotagminIprotein.
Theantibodyispreparedfrompooledrabbitserumbyaffinitypurificationusingacolumntowhichthepeptideimmunogenwascoupled.
Synaptotagmin1isasynapticvesiclemembraneglycoproteinthatiswidelyexpressedthroughouttheCNSandisgenerallythoughttoactastheCa2+sensorintheregulationofexocytosisandneurotransmitterrelease(LittletonandBellen1995).Recentstudiesindicatethatsynaptotagmin’sCa2+mediatedbindingofSNAP25isessentialfortheCa2+dependenttriggeringofmembranefusion(Zhangetal.,2002).Ithasrecentlybeendemonstratedthatdiscreteresidueswithinthec(2)bbindingdomainofsynaptotagmin1independentlyspecifyendocyticrateandsynapticvesiclesize(Poskanzeretal.,2006).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgBSApermland50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
LittletonJT,BellenHJ(1995)SynaptotagmincontrolsandmodulatessynapticvesiclefusioninaCa(2+)dependentmanner.TrendsNeurosci.Apr;18(4):177-83.
ZhangX,Kim-MillerMJ,FukudaM,KowalchykJA,MartinTF(2002)Ca2+dependentsynaptotagminbindingtoSNAP25isessentialforCa2+triggeredexocytosis.NeuronMay16;34(4):599-611.
PoskanzerKE,FetterRD,DavisGW(2006)Discreteresiduesinthec(2)bdomainofsynaptotagmin1independentlyspecifyendocyticrateandsynapticvesiclesize.NeuronApr6;50(1):49-62.
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。