Mousemonoclonalantibody
Theantigenisasynapticimmunoprecipitate(crude)fromhumanbrain.
TheantibodyisProteinGpurifiedculturesupernatant.
Syntaxinsareafamilyofproteinsinvolvedinthedockingofsynapticvesicleswiththeplasmamembrane.Syntaxin,alongwithsynaptobrevinandSNAP-25,formstheSNAREcomplexwhichmediatessynapticvesiclefusionandexocytosis.BindingofMunc18-1totheN-terminusofsyntaxin1hasbeendemonstratedtobeessentialforexocyticmembranefusion(Khvotchevetal.,2007).Decreasedlevelsofphosphorylatedsyntaxin1intheprefrontalcortexhaverecentlybeencorrelatedwithschizophrenia(Castilloetal.,2010).
100µlin10mMHEPES(pH7.5),150mMNaCl,100µgpermlBSAand50%glycerol.Adequateamountofmaterialtoconduct10-miniWesternBlots.
Forlongtermstorage–20°Cisrecommended.Stableat–20°Cforatleast1year.
KhvotchevM,DulubovaI,SunJ,DaiH,RizoJ,SüdhofTC(2007)DualmodesofMunc18-1/SNAREinteractionsarecoupledbyfunctionallycriticalbindingtosyntaxin-1Nterminus.JNeurosci.2007Nov7;27(45):12147-55
CastilloMA,GhoseS,TammingaCA,Ulery-ReynoldsPG(2010)Deficitsinsyntaxin1phosphorylationinschizophreniaprefrontalcortex.BiolPsychiatry.67(3):208-16
Honer,W.G.,Hu,L.&Davies,P.Humansynapticproteinswithaheterogeneousdistributionincerebellumandvisualcortex.BrainResearch609,9-20(1993).
PhosphoSolutions 由 Drs 于 2001 年创立。Michael D. Browning、John W. Haycock 和 Andrew J. Czernik,他们在 Paul Greengard 博士的实验室共同工作,Paul Greengard 博士因研究蛋白质磷酸化在信号转导中的作用而共同获得 2000 年诺贝尔医学奖在神经系统中。这种专业知识使我们主要关注神经科学领域中感兴趣的蛋白质。我们位于科罗拉多州奥罗拉的 Fitzsimons 生命科学园区,是一支敬业、友好、勤奋的科学家团队,致力于为您提供最优质的抗体和业内客户支持。