Antigen/Purification: Collapse
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser831 of GluR1.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Biological Significance: Collapse
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is potentiated by phosphorylation at Ser831 which has been shown to be mediated by either PKC or CaM kinase II (McGlade-McCulloh et al., 1993; Mammen et al., 1999; Roche et al., 1996). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Soderling and Derkach, 2000).
Storage
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.
Product Specific References
Lameth, J., Gervais, A., Colin, C., Lévêque, P., Jay, T.M., Edeline, J.M. and Mallat, M., 2017. Acute Neuroinflammation Promotes Cell Responses to 1800 MHz GSM Electromagnetic Fields in the Rat Cerebral Cortex. Neurotoxicity Research, pp.1-16. PMID: 28578480
O’Leary, H., Bernard, P. B., Castano, A. M., & Benke, T. A. (2016). Enhanced long term potentiation and decreased AMPA receptor desensitization in the acute period following a single kainate induced early life seizure. Neurobiology of disease, 87, 134-144. PMID: 26706598
Xue, B., Chen, E. C., He, N., Jin, D. Z., Mao, L. M., & Wang, J. Q. (2016). Integrated regulation of AMPA glutamate receptor phosphorylation in the striatum by dopamine and acetylcholine. Neuropharmacology. PMID: 27060412
Mao, L. M., Xue, B., Jin, D. Z., & Wang, J. Q. (2015). Dynamic increases in AMPA receptor phosphorylation in the rat hippocampus in response to amphetamine. Journal of neurochemistry. Jun;133(6):795-805. PMID: 25689263
Pasek, J. G., Wang, X., & Colbran, R. J. (2015). Differential CaMKII regulation by voltage-gated calcium channels in the striatum. Molecular and Cellular Neuroscience, 68, 234-243. PMID: 26255006
Mao, L. M., Hastings, J. M., Fibuch, E. E., & Wang, J. Q. (2014). Propofol selectively alters GluA1 AMPA receptor phosphorylation in the hippocampus but not prefrontal cortex in young and aged mice. European journal of pharmacology, 738, 237-244. PMID: 24907515
Xue, B., Edwards, M. C., Mao, L. M., Guo, M. L., Jin, D. Z., Fibuch, E. E., & Wang, J. Q. (2014). Rapid and sustained GluA1 S845 phosphorylation in synaptic and extrasynaptic locations in the rat forebrain following amphetamine administration. Neurochemistry international, 64, 48-54. PMID: 24231469
Barcomb, K., Coultrap, S. J., & Bayer, K. U. (2013). Enzymatic activity of CaMKII is not required for its interaction with the glutamate receptor subunit GluN2B. Molecular pharmacology, 84(6), 834-843. PMID: 24056996
Yuxia Jiao, Nidhi Jalan-Sakrikar, A. J. Robison, Anthony J. Baucum, II, Martha A. Bass, and Roger J. Colbran (2011) Characterization of a Central Ca2+/Calmodulin-dependent Protein Kinase IIα/β Binding Domain in Densin That Selectively Modulates Glutamate Receptor Subunit Phosphorylation. J. Biol. Chem. 286: 24806 – 24818. PMID: 21610080
Steven J. Coultrap, Isabelle Buard, Jaqueline R. Kulbe, Mark L. Dell’Acqua, and K. Ulrich Bayer (2010) CaMKII Autonomy Is Substrate-dependent and Further Stimulated by Ca2+/Calmodulin. J. Biol. Chem., 285: 17930 – 1793. PMID: 20353941
Robert M. Sears, Rong-Jian Liu, Nandakumar S. Narayanan, Ruth Sharf, Mark F. Yeckel, Mark Laubach, George K. Aghajanian, and Ralph J. DiLeone (2010) Regulation of Nucleus Accumbens Activity by the Hypothalamic Neuropeptide Melanin-Concentrating Hormone J. Neurosci., 30: 8263 – 8273. PMID: 20554878
Kurtis D. Davies, Susan M. Goebel-Goody, Steven J. Coultrap, and Michael D. Browning (2008) Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not α-Amino-3-hydroxy-5-methyl-4- isoxazolepropionic Acid (AMPA) Receptor Internalization J. Biol. Chem., 283: 33138 – 33146. PMID: 18819923
Sergio Leal-Ortiz, Clarissa L. Waites, Ryan Terry-Lorenzo, Pedro Zamorano, Eckart D. Gundelfinger, and Craig C. Garner (2008) Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis. J. Cell Biol., 181: 831 – 846. PMID: 18519737
